Wayne State University

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School of Medicine

Biochemistry

Sharon Ackerman

Sharon Ackerman

Associate Professor

sackerm@med.wayne.edu

313-577-8645

Sharon Ackerman

Position Title

 Associate Professor

Education

Ph.D., New York University, NY, NY, 1987

Office Location

4213 Scott Hall

Research

Dr. Ackerman's research involves mitochondrial oxidative phosphorylation with emphasis on the ATP synthase enzyme.

Publications

  • Sellem, CH, Di Rago, JP, Lasserre, JP, Sharon H Ackerman, Sainsard-Chanet, A: “Regulation of Aerobic Energy Metabolism in Podospora anserina by Two Paralogous Genes Encoding Structurally Different c-Subunits of ATP Synthase”, PLoS Genet., 12: e1006161, 2016.
  • Clark GW, Ackerman SH, Tillier ER, Gatti DL: “Multidimensional mutual information methods for the analysis of covariation in multiple sequence alignments”, BMC Bioinformatics, 15:157, 2014.
  • Tetaud E, Godard F, Giraud MF, Ackerman SH, di Rago JP: “The depletion of F₁ subunit ε in yeast leads to an uncoupled respiratory phenotype that is rescued by mutations in the proton- translocating subunits of F₀”, Mol Biol Cell, 6:791-9, 2014.
  • Kabala AM, Lasserre JP, Ackerman SH, di Rago JP, Kucharczyk R: “Defining the impact on yeast ATP synthase of two pathogenic human mitochondrial DNA mutations, T9185C and T9191C”, Biochimie, 100:200-6, 2014.
  • Ackerman, SH, and Gatti, DL: Biapenem inactivation by B2 metallo β-lactamases: energy landscape of the hydrolysis reaction, PLoS One 8(1):e55136, 2013.
  • Ackerman SH, Tillier ER, Gatti DL: Accurate simulation and detection of coevolution signals in multiple sequence alignments, PLoS One 7(10):e47108, 2012.
  • Ackerman SH, Gatti DL: The contribution of coevolving residues to the stability of KDO8P synthase, PLoS One. 6(3):e17459, 2011.
  • Kucharczyk R, Ezkurdia N, Couplan E, Procaccio V, Ackerman SH, Blondel M, di Rago JP: Consequences of the pathogenic T9176C mutation of human mitochondrial DNA on yeast mitochondrial ATP synthase, Biochim Biophys Acta.- Bioenergetics, 1797(6-7):1105-12, 2010.
  • Meulemans A, Seneca S, Pribyl T, Smet J, Alderweirldt V, Waeytens A, Lissens W, Van Coster R, De Meirleir L, di Rago JP, Gatti DL, Ackerman SH: Defining the pathogenesis of the human Atp12p W94R mutation using a Saccharomyces cerevisiae yeast model, J Biol Chem. 285(6):4099-109, 2010.
  • Ludlam A, Brunzelle J, Pribyl T, Xu X, Gatti DL, Ackerman SH: Chaperones of F1- ATPase, J Biol Chem. 284(25):17138-46, 2009.
  • Ackerman SH, Tzagoloff A: Methods to determine the status of mitochondrial ATP synthase assembly, Methods Mol Biol. 372:363-77, 2007.
  • Ackerman SH, Tzagoloff A: Function, structure, and biogenesis of mitochondrial ATP synthase, Prog Nucleic Acid Res Mol Biol. 80:95-133, 2005.
  • Lefebvre-Legendre L, Salin B, Schaëffer J, Brèthes D, Dautant A, Ackerman SH, di Rago JP: Failure to assemble the alpha 3 beta 3 subcomplex of the ATP synthase leads to accumulation of the alpha and beta subunits within inclusion bodies and the loss of mitochondrial cristae in Saccharomyces cerevisiae, J Biol Chem. 280(18):18386-92, 2005.
  • Hinton A, Gatti DL, Ackerman SH: The molecular chaperone, Atp12p, from Homo sapiens. In vitro studies with purified wild type and mutant (E240K) proteins, J Biol Chem. 279(10):9016-22, 2004.
  • Hinton A, Zuiderweg ER, Ackerman SH: A purified subfragment of yeast Atp11p retains full molecular chaperone activity, J Biol Chem. 278(36):34110-3, 2003.
  • Barrientos A, Korr D, Barwell KJ, Sjulsen C, Gajewski CD, Manfredi G, Ackerman S, Tzagoloff A: MTG1 codes for a conserved protein required for mitochondrial translation, Mol Biol Cell. 14(6):2292-302, 2003.
  • Ackerman SH: Atp11p and Atp12p are chaperones for F(1)-ATPase biogenesis in mitochondria, Biochim Biophys Acta. 1555(1-3):101-5, 2002.
  • Sheluho D, Ackerman SH: An accessible hydrophobic surface is a key element of the molecular chaperone action of Atp11p, J Biol Chem. 276(43):39945-9, 2001.
  • Wang ZG, White PS, Ackerman SH: Atp11p and Atp12p are assembly factors for the F(1)- ATPase in human mitochondria, J Biol Chem. 276(33):30773-8, 2001.
  • Wang ZG, *Sheluho D, Gatti DL, Ackerman SH: The alpha-subunit of the mitochondrial F(1) ATPase interacts directly with the assembly factor Atp12p, EMBO J. 19(7):1486-93, 2000.
  • Wang ZG, Ackerman SH: The assembly factor Atp11p binds to the beta-subunit of the mitochondrial F(1)-ATPase, J Biol Chem. 275(8):5767-72, 2000.
  • Wang ZG, Schmid KJ, Ackerman SH: The Drosophila gene 2A5 complements the defect in mitochondrial F1-ATPase assembly in yeast lacking the molecular chaperone Atp11p, FEBS Lett. 452(3):305-8, 1999.
  • Gatti DL, Tarr G, Fee JA, Ackerman SH: Cloning and sequence analysis of the structural gene for the bc1-type Rieske iron-sulfur protein from Thermus thermophilus HB8, J Bioenerg Biomembr. 30(3):223-33, 1998.
  • Wang ZG, Ackerman SH: Mutational studies with Atp12p, a protein required for assembly of the mitochondrial F1-ATPase in yeast. Identification of domains important for Atp12p function and oligomerization, J Biol Chem. 273(5):2993-3002, 1998.
  • Liang Y, Ackerman SH: Characterization of mutations in the beta subunit of the mitochondrial F1-ATPase that produce defects in enzyme catalysis and assembly, J Biol Chem. 271(43):26522-8, 1996.
  • Wang ZG, Ackerman SH: Identification of functional domains in Atp11p. Protein required for assembly of the mitochondrial F1-ATPase in yeast, J Biol Chem. 271(9):4887-94, 1996.
  • White M, Ackerman SH: Bacterial production and characterization of ATP11, a yeast protein required for mitochondrial F1-ATPase assembly, Arch Biochem Biophys. 319(1):299- 304, 1995.
  • Paul MF, Ackerman S, Yue J, Arselin G, Velours J, Tzagolof A: Cloning of the yeast ATP3 gene coding for the gamma-subunit of F1 and characterization of atp3 mutants, J Biol Chem. 269(42):26158-64, 1994.
  • Ackerman SH, Martin J, Tzagoloff A: Characterization of ATP11 and detection of the encoded protein in mitochondria of Saccharomyces cerevisiae, J Biol Chem. 267(11):7386-94, 1992.
  • Vambutas A, Ackerman SH, Tzagoloff A: Mitochondrial translational-initiation and elongation factors in Saccharomyces cerevisiae, Eur J Biochem. 201(3):643-52, 1991.
  • Bowman S, Ackerman SH, Griffiths DE, Tzagoloff A: Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase, J Biol Chem. 266(12):7517-23, 1991.
  • Ackerman SH, Gatti DL, Gellefors P, Douglas MG, Tzagoloff A: ATP13, a nuclear gene of Saccharomyces cerevisiae essential for the expression of subunit 9 of the mitochondrial ATPase, FEBS Lett. 278(2):234-8, 1991.
  • Ackerman SH, Tzagoloff A: Identification of two nuclear genes (ATP11, ATP12) required for assembly of the yeast F1-ATPase, Proc Natl Acad Sci U S A. 87(13):4986-90, 1990.
  • Ackerman SH, Tzagoloff A: ATP10, a yeast nuclear gene required for the assembly of the mitochondrial F1-F0 complex, J Biol Chem. 265(17):9952-9, 1990. PMID: 2141026
  • Coleman PS, Ackerman S: Catalytic Cooperativity in F1-ATPase: Photoaffinity Labeling Studies with BzATP in Molecular Structure, Function, and Assembly of the ATP Synthases, Springer, 239-249, 1989.
  • Ackerman SH, Grubmeyer C, Coleman PS: Evidence for catalytic cooperativity during ATP hydrolysis by beef heart F1-ATPase. Kinetics and binding studies with the photoaffinity label BzATP, J Biol Chem. 262(28):13765-72, 1987.
  • Williams N, Ackerman SH, Coleman PS: Benzophenone-ATP: a photoaffinity label for the active site of ATPases. Methods Enzymol. 126:667-82, 1986.

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