Education

Ph.D., Marburg (Germany), 1999

Laboratory Web Site

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Research

Dr. Huttemann's research includes cytochrome c and cytochrome c oxidase, the regulation of mitochondrial oxidative phosphorylation through cell signaling pathways, and mitochondria as a target for therapy and diagnostics in human diseases including ischemia reperfusion injury in the heart and brain and cancer.

Research Focus

Our team studies mitochondrial function using genetic and biochemical approaches. We focus on two key components of the mitochondrial oxidative phosphorylation machinery, cytochrome c oxidase (COX) and the small electron carrier cytochrome c (Cytc). COX is the terminal enzyme of the mitochondrial respiratory chain, "burns" the oxygen we breathe to water, and pumps protons across the inner mitochondrial membrane generating the mitochondrial membrane potential, which is utilized by ATP synthase to produce energy in the form of ATP. Cytc has two distinct functions: it delivers electrons to COX, but it also participates in programmed cell death (apoptosis).

Publications

• Mahapatra G, Varughese A, Ji Q, Lee I, Liu J, Vaishnav A, Sinkler C, Kapralov AA, Moraes CT, Sanderson TH, Stemmler TL, Grossman LI, Kagan VE, Brunzelle JS, Salomon AR, Edwards BF, Hüttemann M (2017) Phosphorylation of Cytochrome c Threonine 28 Regulates Electron Transport Chain Activity in Kidney: IMPLICATIONS FOR AMP KINASE. J Biol Chem 292: 64-79.
• Aras S, Arrabi H, Purandare N, Hüttemann M, Kamholz J, Zuchner S, Grossman LI (2017) Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration. Biochim Biophys Acta 1864: 440-448.
• Zhang K, Wang G, Zhang X, Hüttemann PP, Qiu Y, Liu J, Mitchell A, Lee I, Zhang C, Lee JS, Pecina P, Wu G, Yang ZQ, Huttemann M, Grossman LI (2016) COX7AR is a Stress-inducible Mitochondrial COX Subunit that Promotes Breast Cancer Malignancy. Sci Rep 6: 31742.
• Lee I, Hüttemann M, Malek MH (2016) (-)-Epicatechin Attenuates Degradation of Mouse Oxidative Muscle Following Hindlimb Suspension. J Strength Cond Res 30: 1-10.
• Diedrich JD, Rajagurubandara E, Herroon MK, Mahapatra G, Hüttemann M, Podgorski I (2016) Bone marrow adipocytes promote the warburg phenotype in metastatic prostate tumors via HIF-1alpha activation. Oncotarget 7: 64854-64877.
• Shay J, Elbaz HA, Lee I, Zielske SP, Malek MH, Hüttemann M (2015) Molecular mechanisms and therapeutic effects of (-)-epicatechin and other polyphenols in cancer, inflammation, diabetes, and neurodegeneration. Oxid Med Cell Longev in press.
• Maurer SF, Fromme T, Grossman LI, Hüttemann M, Klingenspor M (2015) The brown and brite adipocyte marker Cox7a1 is not required for non-shivering thermogenesis in mice. Sci Rep 5: 17704.
• Marshall J, Wong KY, Rupasinghe CN, Tiwari R, Zhao X, Berberoglu ED, Sinkler C, Liu J, Lee I, Parang K, Spaller MR, Hüttemann M, Goebel DJ (2015) Inhibition of N-Methyl-D-aspartate-induced Retinal Neuronal Death by Polyarginine Peptides Is Linked to the Attenuation of Stress-induced Hyperpolarization of the Inner Mitochondrial Membrane Potential. J Biol Chem 290: 22030-22048.
• Kadenbach B, Hüttemann M 2015) The subunit composition and function of mammalian cytochrome c oxidase. Mitochondrion 24: 64-76.
• Horsch M, Aguilar-Pimentel JA, Bonisch C, Come C, Kolster-Fog C, Jensen KT, Lund AH, Lee I, Grossman LI, Sinkler C, Hüttemann M, Bohn E, Fuchs H, Ollert M, Gailus-Durner V, de Angelis MH, Beckers J (2015) Cox4i2, Ifit2, and Prdm11 Mutant Mice: Effective Selection of Genes Predisposing to an Altered Airway Inflammatory Response from a Large Compendium of Mutant Mouse Lines. PLoS One 10: e0134503.
• Elbaz HA, Lee I, Antwih DA, Liu J, Hüttemann M, Zielske SP (2014) Epicatechin stimulates mitochondrial activity and selectively sensitizes cancer cells to radiation. PloS One 9: e88322.
• Sanderson TH, Mahapatra G, Pecina P, Ji Q, Yu K, Sinkler C, Varughese A, Kumar R, Bukowski MJ, Tousignant RN, Salomon AR, Lee I, Hüttemann M (2013) Cytochrome c is tyrosine 97 phosphorylated by neuroprotective insulin treatment. PLoS One 8: e78627.
• Lee I, Hüttemann M, Liu J, Grossman LI, Malek MH (2012) Deletion of heart-type cytochrome c oxidase subunit 7a1 impairs skeletal muscle angiogenesis and oxidative phosphorylation. J Physiol 590: 5231-5243.
• Hüttemann M, Lee I, Gao X, Pecina P, Pecinova A, Liu J, Aras S, Sommer N, Sanderson TH, Tost M, Neff F, Aguilar-Pimentel JA, Becker L, Naton B, Rathkolb B, Rozman J, Favor J, Hans W, Prehn C, Puk O, Schrewe A, Sun M, Hofler H, Adamski J, Bekeredjian R, Graw J, Adler T, Busch DH, Klingenspor M, Klopstock T, Ollert M, Wolf E, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, Weissmann N, Doan JW, Bassett DJ, Grossman LI (2012) Cytochrome c oxidase subunit 4 isoform 2-knockout mice show reduced enzyme activity, airway hyporeactivity, and lung pathology. FASEB J 26: 3916-3930.
• Hüttemann M, Klewer S, Lee I, Pecinova A, Pecina P, Liu J, Lee M, Doan JW, Larson D, Slack E, Maghsoodi B, Erickson RP, Grossman LI (2012) Mice deleted for heart-type cytochrome c oxidase subunit 7a1 develop dilated cardiomyopathy. Mitochondrion 12: 294-304.

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